Sperm vitality assessment, one of the basic tools used in semen analysis, is a key element in the success of IVF. Sperm VitalStain, which contains both eosin and nigrosin, is a key component of Nidacon's optimized sperm vitality staining technique. Our technique is based on the principle that dead cells (i.e. those with damaged plasma membranes) absorb eosin and show a red coloration. Nigrosine is used as a counter-stain to facilitate visualization of unstained (white) living cells.
Features
SpermVitalStain uses the one-step eosin-nigrosin technique to determine the percentage of live spermatozoa. It is based on the principle that dead cells (i.e. those with damaged plasma membranes) absorb eosin and stain red. Nigrosine provides the background to facilitate visualization of the unstained (white) living cells.