PureSperm 40, PureSperm 80 and PureSperm 90 offer all the benefits of a PureSperm 100 gradient, with the added advantage of already being diluted, saving preparation time. PureSperm 40, PureSperm 80 and PureSperm 90 are formulated to minimize sudden changes in pH and osmolarity during the transition of sperm from the semen sample to the fertilization medium. This prevents premature hyperactivation and improves fertilization potential.
Features
- PureSperm 40, PureSperm 80 and PureSperm 90 are formulated to minimize sudden changes in pH and osmolarity during the transition of sperm from a semen sample to the fertilization medium. This prevents premature hyperactivation and improves fertilization potential.
- PureSperm 40, PureSperm 80 and PureSperm 90 maintain a pH between 7.4 and 7.8 at room temperature (17 to 27°C) and when incubated at 37°, providing conditions conducive to good sperm motility, survival time and fertilization potential.
- These preparations contain glucose as an energy substrate for normal cell metabolism and sperm function.
- The gradient separates motile spermatozoa from extracellular cells and seminal plasma. In addition, damage to sperm DNA and structural molecules by reactive oxygen species (ROS) is minimized by efficient removal of immature sperm and lymphocytes. Bacteria, epithelial cells and cellular debris are also separated from motile spermatozoa, which can be difficult to achieve in polysucrose-based gradient preparations.